Device

Part:BBa_K119010:Design

Designed by: Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja,Martin del castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, E   Group: iGEM08_LCG-UNAM-Mexico   (2008-10-01)


LuxR_cI_aiiA_Normal lacZ promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2005
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1006


Design Notes

We needed a repressor which could be induced by a "signal" in order to inhibit the RcnA(BBa_K119003, BBa_K119009) activity. We achieved this by taking advantage of the LuxR promoter present in the Chiba device BBa_I729006. So, we placed cI downstream of this promoter and changed the promoter which was controlling aiiA for the LacZ one.


Source

We based this part in BBa_I729006 from Chiba team

References